Chemiluminescent Western Blot Detection
The FastGene Western ECL Kit is an enhanced chemiluminescent (ECL) substrate based on luminol. It is used to detect horseradish peroxidase (HRP) – conjugated secondary antibodies. The high femtogram or low picogram detection of antigen is enabled by the kits’ brilliant sensitivity and long signal duration. This long chemiluminescent signal duration makes it possible to detect signals on both digital and film-based imaging systems without any loss in signal intensity. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.
- Chemiluminescent substrate based on luminol
- High sensitivity – Detection of antigens with very low concentrations
- Long signal duration – Optimal for digital and film-based imaging
- No optimization required – Easy switch from other brands
- Perfect for PDVF and nitrocellulose membranes
- Compatible with Western Blotting Markers
Mix the luminol solution and peroxide solution in a 1:1 ratio, and thoroughly agitate the chemiluminescent substrate solution well for preparing the 0.1 ml of solution / cm2 of membrane. Place the membrane with the protein side up and remove the membrane from the chemiluminescent substrate solution. Image the membrane with a digital imager.
Comparison between the FastGene Western ECL Kit and competitor products
1000-100 ng BSA were separated in a 4-20% SDS-Page (FastGene Precast Gel) using a primary antibody 1:5000 alpha-BSA (Invitrogen, A11133, corresponding to 4 µg). All ECL kits were used under the same experimental workflow.
An IL-6 fusion protein was detected using a primary antibody against IL-6 from mouse and a secondary anti-mouse peroxide (POD) antibody 2 seconds exposure for the Western Blot. All ECL kits were used under the same experimental workflow.
|1 year at 4°C