Higher yield in a shorter time
Lipopolysaccharides (LPS) are a major constituent of the outer membrane of gram-negative bacteria. The common hot phenol or oil/chloroform/phenol methods for purifying these molecules are both complex and time consuming. The LPS Extraction Kit offers a new approach to extracting LPS with higher yields, fewer steps and in far less time. Based on a robust water/phenol/chloroform extraction technology, the LPS extraction kit is flexible enough to run small culture volumes (<5ml) or large (up to 100 ml) with similar yields.
|Contents||1x 100 ml Lysis Buffer, 1x 80 ml Purification Buffer|
|Number of Purifications||100x 5 ml bacterial cultures|
|Yield||50-100 µg of LPS from a 5ml culture of E. coli (OD600=0.8-1.2)|
|Protocol Time||<45 minutes|
|Protocol Steps||8 steps|
|Storage||>1 year at 4°C|