Literature
Higher yield in a shorter time
Lipopolysaccharides (LPS) are a major constituent of the outer membrane of gram-negative bacteria. The common hot phenol or oil/chloroform/phenol methods for purifying these molecules are both complex and time consuming. The LPS Extraction Kit offers a new approach to extracting LPS with higher yields, fewer steps and in far less time. Based on a robust water/phenol/chloroform extraction technology, the LPS extraction kit is flexible enough to run small culture volumes (<5ml) or large (up to 100 ml) with similar yields.
LPS Extraction Kit (17141)
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SPECIFICATIONS
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| Part Numbers | 17141 |
| Contents | 1x 100 ml Lysis Buffer, 1x 80 ml Purification Buffer |
| Number of Purifications | 100x 5 ml bacterial cultures |
| Yield | 50-100 µg of LPS from a 5ml culture of E. coli (OD600=0.8-1.2) |
| Methodology | Cold phenol/chloroform |
| Protocol Time | <45 minutes |
| Protocol Steps | 8 steps |
| Storage | >1 year at 4°C |