MBP-Trap Affinity Reagents

Capture MBP-tagged Proteins

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Purify MBP-tagged proteins without contaminating peptides
The MBP-Trap is a single chain polypeptide antibody coupled to agarose beads for immunoprecipitation of MBP-tagged proteins. All Nanobody-Traps use a recombinant antibody fragment lacking a heavy chain. For this reason the MBP-Trap cannot dissociate from the agarose bead carrier, and therefore, offers the cleanest pulldown results. The MBP-Trap recognizes E.coli Maltose binding protein (MBP) which is often used as a fusion protein to increase solubility and expression level of a protein. Furthermore, MBP- tagged fusion proteins are often used in protein-protein interaction studies. Both approaches require highly specific tools to isolate and detect MBP fusion proteins. MBP is that tool–we guarantee you’ll see the fastest and most efficient performance! Typical results yield much lower background than other commercial or home-made affinity reagents. This makes the MBP-Trap perfect for labs that need crystal-clean results for applications like mass spectrometry or other persnickety analyses.

Better than traditional monoclonal antibodies
To the right is a pull-down experiment showing the MBP-Trap technology used during an immunoprecipitation of Maltose-binding protein from an E. colicell extract. Compared to amylose, the MBP-Trap binds with a higher specificity to MBP. Therefore, the background is strongly reduced. The pulldown of MBP-fusion proteins is a fast and easy one-step IP that delivers consistent, reproducible data.

Camelidae single-domain antibodies are like IgGs on steroids

The family of animals known as Camelidae (camels, llamas, and alpacas) produce functional antibodies devoid of light chains, so-called "heavy chain" antibodies. These heavy chain antibodies recognize and bind their antigens via a single variable domain. When cleaved from their carboxy tail, these barrel-shaped structures (2x3 nm) are extraordinarily small, naturally-occurring, and intact antigen binding fragments (MW of 13 kDa). These fragments, called Nanobodies, are characterized by high specificity, affinities in the low nanomolar range, and dissociation constants in the sub-nanomolar range (typically 10- to 100-fold better than mouse IgGs). The compact size of Nanobodies makes them extremely stable at temperatures up to 70°C, and functional even in 2M NaCl or 0.5% SDS. These small and powerful antibody fragments can be used in a variety of unique applications. They will open up your research possibilities.

Specificity E-coli
Configuration: Specific Camelidae antibody linked to agarose bead
Part Numbers MBTA010, MBTA020, MBTA100, MBTA200, MBTA400, MBTAK020, MBT250
Particle Size: ~90 µm when coupled to an agarose beads
Storage Buffer: 20% EtOH
Storage and Stability: store at 4°C; stable for one year. Do not freeze.