MEGAquick-spin plus Total Fragment DNA Purification Kit

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The MEGAquick-spin Plus Total Fragment DNA Purification Kit is designed to extract and purify DNA fragments of 65bp – 20kb from normal or low-melt agarose gels in either Tris-acetate-EDTA (TAE) or Tris-borate-EDTA (TBE) buffers. The kit can also purify PCR products directly from a PCR sample (removing primer dimers) and perform DNA clean up from other enzymatic reactions with a recovery of up to 95%. As an added convenience not commonly found in other gel extraction procedures, the agarose gel lysis buffer (BNL Buffer) supplied contains a color indicator that allows easy monitoring of the solution pH for optimal DNA binding. This spin column-based system can bind up to 20 μg of DNA and allows recovery of isolated DNA fragments or PCR products in as little as 10 minutes, depending on the number of samples processed and the protocol used.

Performance – recovery rates

PCR purification and gel extraction were performed for each size fragment (89 bp ~ 20 kb) of PCR amplified products. The blue color shows the product before amplification, and the orange color shows the result after purification. The purification result for each PCR product confirmed 91 to 99% DNA recovery compared to before purification, and in the case of the gel extraction product, 68 to 88% DNA recovery was confirmed compared to the product before purification.

Performance – purification by gel concentration

To check the performance of this kit according to the agarose gel concentration, a small amplified fragment of 89 bp was used. Gel concentrations of 1.5%, 4%, and 5% were applied and each recovery rate result was checked. The recovery rate for each gel concentration was confirmed to be 72% / 61% / 56%.  As a result, it was confirmed that purification of more than 50% was possible even with a 5% high concentration gel.