Myc-Trap Affinity Reagents

Capture Myc-tagged Proteins

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Purify Myc-tagged proteins without contaminating peptides

The Myc-Trap is a single chain polypeptide antibody coupled to agarose beads or magnetic agarose beads for immunoprecipitation of Myc-tagged proteins. All Nanobody-Traps use a recombinant antibody fragment lacking a heavy chain. For this reason the Myc-Trap cannot dissociate from the agarose bead carrier, and therefore offers the cleanest pulldown results. The Myc-Trap recognizes the Myc-tag sequence EQKLISEEDL at the N-terminus, C-terminus, or internal site of any fusion protein. We guarantee you’ll see the fastest and most efficient performance! Typical results yield much lower background than other commercial or home-made affinity reagents. This makes Myc-Trap perfect for labs who need crystal-clean results for applications like mass spectrometry or other persnickety analyses.


Better than traditional monoclonal antibodies

To the right is a pull-down experiment comparing the Myc-Trap technology to a conventional monoclonal Myc antibody. Most researchers today use the immobilized anti-Myc antibody 9E10 for immunoprecipitation of Myc fusion-proteins. During immunoprecipitation with 9E10, heavy and light antibody chains can dissociate from the immobilized 9E10 and contaminate the eluate of the Myc-tagged protein of interest with these antibody molecules. As you can see from the data, the single polypeptide chain used in the Myc-Trap does not suffer from this issue and provides clean results without contaminating peptides.


Camelidae single-domain antibodies are like IgGs on steroids

The family of animals known as Camelidae (camels, llamas, and alpacas) produce functional antibodies devoid of light chains, so-called "heavy chain" antibodies. These heavy chain antibodies recognize and bind their antigens via a single variable domain. When cleaved from their carboxy tail, these barrel-shaped structures (2x3 nm) are extraordinarily small, naturally-occurring, and intact antigen binding fragments (MW of 13 kDa). These fragments, called Nanobodies, are characterized by high specificity, affinities in the low nanomolar range, and dissociation constants in the sub-nanomolar range (typically 10- to 100-fold better than mouse IgGs). The compact size of Nanobodies makes them extremely stable at temperatures up to 70°C, and functional even in 2M NaCl or 0.5% SDS. These small and powerful antibody fragments can be used in a variety of unique applications. They will open up your research possibilities.


MycTraps Coupled to Agarose

Myc-Traps Coupled to Magnetic Agarose Beads

Myc-Traps Non-Coupled

Binding Controls

Spin Columns for agarose beads

SPECIFICATIONS
Configuration: Specific Camelidae antibody linked to agarose bead
Particle Size: ~90 µm when coupled to an agarose beads

~40 µm when coupled to magnetic agarose

Storage Buffer: 20% EtOH
Part Numbers YTA010, YTA020, YTA100, YTA200,YTA400, YTAK020, YTMA010, YTMA020, YGTMA100, YTMA200, YTA400, YTMAK020, YT250
Storage and Stability: store at 4°C; stable for one year. Do not freeze