nBIONIX Hypoxic Cell Culture Kit　

Easy, adaptable and inexpensive hypoxia “chambers”

It’s sometimes imperative for life science researchers to recreate physiological conditions seen by cells – whether to ask important questions about cancer biology, developmental biology, apoptosis, regenerative medicine, cellular biology or a plethora of other biological processes. One condition that can be difficult to replicate is the oxygen-starved, hypoxic, environment associated with tissue damage, tumorigenesis, cellular differentiation and apoptosis.

Until recently, these types of experiments required cultivating cultured cells in a special CO₂ incubator in which an independent supply of carbon dioxide gas and nitrogen gas are necessary to recreate a hypoxic environment for these cells. Such expensive systems have a hard time maintaining hypoxic conditions under 2% O₂ levels.

This is not the case for nBIONIX Hypoxic Cell Culture Kit. These kits enable convenient, precise and inexpensive hypoxic cultivation. It can take less than 10 minutes to create a precisely tuned low oxygen environment from 0.1% to 15% O₂ levels. The gas-barrier pouch contains a precision O₂ meter (to provide continuous feedback), in addition to one or more dishes or plates of cells. This easily transportable micro-environment can be placed in a standard cell culture incubator for long-term incubations under hypoxic conditions.