p53-Trap Affinity Reagents

Novel Binding Proteins for p53 Studies

Request Quote

Analyze p53 in a tube

The tumor suppressor protein p53 plays a major role in cell cycle control, apoptosis, and senescence. Understanding the details of how p53 works within these important cell processes is a focus for many laboratories. One key technique for studying complex cell processes is the pull-down experiment. The p53-Trap is a high quality p53-binding protein coupled to a monovalent matrix (agarose particles) for biochemical analysis of p53 and interacting partners. With the p53-Trap, it is possible to yield ultra-clean results free from contaminants in under 30 minutes. p53-Traps utilize super-high affinity Camelidae antibody fragments that may be used for immuno-precipitation, immuno-purification, and immuno-pull down experiments with up to 10-fold better purity and yield than that of classic mouse monoclonal antibodies. Compatible with a variety of source materials, Nanobody-Traps may be used with mammalian cells, tissues & organs, bacteria, yeast, and even plants. This reagent allows p53 to be perfect candidate for immunoprecipitations, Co-IP, mass spectroscopy, and enzyme activity measurements.


Pulldown of p53 using the p53-Traps

To the right is a Western blot demonstrating the effectiveness of p53 from whole cell extracts. In this case, an immunoprecipitation (IP) of p53 from a protein extract of HEK 293T cells was performed with p53-Trap. Western analysis of the input (I), non-bound flowthrough (FT), and bound (B) fractions were separated by SDS-PAGE followed by Western blotting.


Camelidae single-domain antibodies are like IgGs on steroids

The family of animals known as Camelidae (camels, llamas, and alpacas) produce functional antibodies devoid of light chains, so-called "heavy chain" antibodies. These heavy chain antibodies recognize and bind their antigens via a single variable domain. When cleaved from their carboxy tail, these barrel-shaped structures (2x3 nm) are extraordinarily small, naturally-occurring, and intact antigen binding fragments (MW of 13 kDa). These fragments, called Nanobodies, are characterized by high specificity, affinities in the low nanomolar range, and dissociation constants in the sub-nanomolar range (typically 10- to 100-fold better than mouse IgGs). The compact size of Nanobodies makes them extremely stable at temperatures up to 70°C, and functional even in 2M NaCl or 0.5% SDS. These small and powerful antibody fragments can be used in a variety of unique applications. They will open up your research possibilities.


P53 N-Terminus-Traps Coupled to Agarose

P53 C-Terminus-Traps Coupled to Agarose

Binding Controls

Spin Columns for agarose beads

SPECIFICATIONS
Configuration:

p53-TrapA

Specific Camelidae antibody linked to agarose bead. Two versions: N-terminus and C-terminus
Part Numbers
PTA010, PTA020, PTA100, PTA200, PTA400, PTAK020, PTA210, PTA220, PTA2100, PTA2200, PTA2400, PTAK220

p53-Trap

Uncoupled and purified Camelidae antibody
Part Numbers
PTA250, PTA2250
Particle Size:

p53-Trap A

~90 µm when coupled to an agarose beads

p53-Trap

No particle coupled
Storage Buffer:

p53-Trap A

20% EtOH

p53-Trap

1x PBS; Preservative: 0.01% Sodium Azide
Storage and Stability:

p53-Trap A

store at 4°C; stable for one year. Do not freeze.

p53-Trap

store at 4°C; stable for one year. Do not freeze.