ProofDog Hi-Fi DNA Polymerase

A perfect blend of performance and fidelity
ProofDog Hi-Fi is a recombinantly-engineered DNA polymerase with a proof-reading domain and advanced processivity domain. This combination provides ultra-accurate nucleotide incorpration (>50X the fidelity of Taq polymerase) with speeds and robustness that greatly exceed that of native Taq polymerase. Simply put, ProofDog does it all, and a fraction of the cost compared to other brand name polymerases.

ProofDog HiFi is a great option for even challenging applications like Next Generation Sequencing. The figure below demonstrates that ProofDog HiFi amplifies NGS libraries with sufficient accuracy  for high throughput sequencing and alignment to a reference genome that is comparable to other high-end polymerases.

Made for challenging PCR applications
Starting with a fundamental improvement to both processivity and yield, ProofDog HiFi performs vigorously across a broad range of PCR conditions . Faster processing ensures that PCR is performed at maximum efficiency even when time-limited elongation steps are not fully optimized. Higher yields are the result of more full length amplicons over the course of every cycle of a PCR protocol, regardless of the conditions.

ProofDog HiFi is also incredibly resistant to common types of PCR inhibitors found in crude DNA extracts. For example, high salt concentrations have an ameliorated effect on this polymerase’s activity.

Which Bulldog Bio polymerase is best for me?
We have four different options for PCR enzymes; three Taq-based polymerases and one proof-reader. Choose the one that’s best for your experiments. First, check out this quick reference of key features:

Next, check to see which factors are most important for your PCR experiment: