TaqDog DNA Polymerases

Premium and Hot Start PCR enzymes for every lab

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Guaranteed results with amplified savings
TaqDog Premium is a great choice for your everyday PCR needs. This recombinant DNA polymerase is derived from Thermus aquaticus is perfect for 3-step, 2-step, and touchdown protocols, as well as completely compatible with common SYBR™ Green-based qPCR buffers. Each kit includes 10x reaction buffer and is optimized for improved amplification efficiency. Better efficiency allows many protocols to be completed in under 1 hour — that is if your thermal cycler can keep up!  Though not a proof-reading polymerase, TaqDog Premium can precisely amplify DNA fragments between 50 bp and 5,000 bp.

TaqDog Premium is also a great enzyme to consider for dramatically reducing the cost of your qPCR experiments. Starting at only a few pennies per unit, its ideal for labs performing mouse tail genotyping, and other high volume PCR processing. The purity of TaqDog Premium is so good, that it’s still 100% fully active after 14 days at 37°C. That’s why we ship it at room temperature — a cost savings for your lab and waste savings for the environment.

 


Ready for all of your PCR needs
TaqDog PCR enzymes can be used in many types of experiments that require a robust and active Taq polymerase. It’s highly stable, and able to be diluted, making it perfect for labs that are looking for a reliable and cost effective PCR enzyme.


New hot start TaqDog leads to cleaner PCR
Hot start PCR has been around for over 20 years, and has been conclusively shown to reduce amplification artifacts caused by low-temperature “false”-priming. Many methods have been developed over this time, but TaqDog Hot Start’s aptamer technology combines their best features while eliminating their worst drawbacks. It’s based upon a DNA construct engineered to form a secondary hairpin structure that reversibly-binds Taq at room temperature. When bound, the polymerase activity is inhibited at temperature at or below 45 °C. This prevents the extension of incorrectly primed regions in a sample’s template DNA. At higher temperatures, the aptamer falls off, and only strongly binding, and specific, primers can then initiate polymerization. This results in final PCR products that are clear of extra, unwanted bands, including the notoriously aggravating primer-dimer bands.

TaqDog Hot Start aptamers are synthetically prepared and HPLC purified for both a precise and defined composition which greatly reduces lot-to-lot variation. This also avoids contamination with trace amounts of mammalian DNA or cellular contents that can be associated with antibody preps. TaqDog Hot Start aptamers are inert and immune to proteases. They contain a 3′-cap that preventing their amplification during PCR or even ability to be cloned. Interference of aptamers with downstream applications has not been observed. Moreover, DNA aptamers do not become irreversibly denatured over time (due to high temperatures), but in contrast, allow for more robust amplification and higher yields. Unlike antibody-mediated inhibition, aptamers are able to re-bind and inhibit our polymerase after the completion of a PCR protocol. This is useful in applications where baseline signal is critical and even modest post-PCR activity (for example during long long-temperature “soak” steps common to many protocols) can lead to artifacts in data.


It ain’t rocket science any more

PCR used to be considered an art, but in the 30 years since its general acceptance into scientific experimentation it’s become one of the best-characterized bio-enzymatic processes. To this end, we ask you this simple question: why pay prices that don’t reflect this fact? All of Bulldog Bio’s PCR products are priced fairly and promise satisfying results. It’s that simple.


SPECIFICATIONS
Species of Orgin Thermus aquaticus
Enzyme Type Thermostable DNA polymerase
Form Recombinant – Engineered
Part Numbers TQDG010, TQDG050, TQDG100, TQDG500, HTQDG010, HTQDG050, HTQDG100, HTQDG500
5’–3′ Exonuclease Activity Yes
“A” Overhang Yes
3’–5′ Exonuclease Activity No
Enzyme Concentration 5 units/µL
Reaction Buffer (included) Yes. 10x concentration (no dNTPs)
GC Enhancer Yes
Storage 1 year at -20°C
Shipping Method Room Temperature. Stable for at least 3 weeks even at 37°C
DNase Free Yes
RNase Free Yes
Protease Free Yes
SYBR™ Green Compatible Yes
Hot Start Yes.  Hot Start version is aptamer based