V5-Trap Affinity Reagents

Novel Binding Protein for V5 Studies

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Analyze V5 fusion proteins in a tube

The V5-tag is a popular, well-established peptide tag used by many laboratories. With the V5-Trap, yields of fusion proteins purification are high and free from heavy and light-chain contaminants, all in under 30 minutes. Save time, save effort and get cleaner results.

The secret to V5-Traps success are the use of super-high affinity Camelidae antibody fragments called Nanobodies. V5-Traps may be used for immuno-precipitation, immuno-purification and immuno-pull down experiments with up to 10-fold better purity and yield than that of classic mouse monoclonal antibodies. Compatible with a variety of source materials, Nanobody-Traps may be used with mammalian cells, tissues & organs, bacteria, yeast and even plants. These reagents allow your mNeonGreen-fusions to be perfect candidates for immunoprecipitations, Co-IP, mass spectroscopy, and enzyme activity measurements.


V5-Trap for immunoprecipitations

Immunoprecipitations (IP) of V5-tagged protein extracts of V5-expressing E.coli cells.  Input (I), non-bound (FT) and bound (B) fractions were separated by SDS-PAGE followed by Coomassie staining and Western Blotting. Unlike conventional antibodies, the mNeonGreen-Trap is free from heavy and/or light chain contaminants and therefore yields cleaner results.


IP from different cell lysates without V5-tagged proteins => The V5-Trap has very low background


Camelidae single-domain antibodies are like IgGs on steroids

The family of animals known as Camelidae (camels, llamas, and alpacas) produce functional antibodies devoid of light chains, so-called "heavy chain" antibodies. These heavy chain antibodies recognize and bind their antigens via a single variable domain. When cleaved from their carboxy tail, these barrel-shaped structures (2x3 nm) are extraordinarily small, naturally-occurring, and intact antigen binding fragments (MW of 13 kDa). These fragments, called Nanobodies, are characterized by high specificity, affinities in the low nanomolar range, and dissociation constants in the sub-nanomolar range (typically 10- to 100-fold better than mouse IgGs). The compact size of Nanobodies makes them extremely stable at temperatures up to 70°C, and functional even in 2M NaCl or 0.5% SDS. These small and powerful antibody fragments can be used in a variety of unique applications. They will open up your research possibilities.


V5-Traps Coupled to Agarose

V5-Traps Coupled to Magnetic Agarose Beads

V5-Traps Non-Coupled

Binding Controls

Spin Columns for agarose beads

SPECIFICATIONS
Specificity:

All V5-Traps

V5-tag sequence motif GKPIPNPLLGLDST

Binding Capacity

6 µg/10µl slurry (agarose)

7 µg/10µl slurry (magnetic-agarose)

Configuration:

V5-Trap-Trap A

Specific Camelidae antibody linked to agarose bead
Part Numbers
V5TA010, V5TA020, V5TA100, V5TA200, V5TA400, V5TAK020

V5-Trap MA

Specific Camelidae antibody linked to magnetic agarose particle
Part Numbers
V5TMA010, V5TMA020, V5TMA100, V5TMA200, V5TMA400, V5TMAK020

V5-Trap

Uncoupled and purified Camelidae antibody
Part Numbers
V5T250
Particle Size:

V5-Trap A

~90 µm when coupled to an agarose bead

V5-Trap MA

~40 µm when coupled to a magnetic-agarose bead

V5-Trap

No particle coupled
Storage Buffer:

V5-Trap A

20% EtOH

V5-Trap MA

20% EtOH
Storage and Stability:

V5-Trap A

store at 4°C; stable for one year. Do not freeze.

V5-Trap MA

store at 4°C; stable for one year. Do not freeze.

V5-Trap

store at 4°C; stable for one year. Do not freeze.