LOCUS pSpot2 2673 bp DNA circular UNA 16-OCT-2017 DEFINITION Expression plasmid for C-term. Spot-tag fusion proteins, E. coli ACCESSION ev-2 VERSION KEYWORDS . SOURCE ORGANISM REFERENCE 1 AUTHORS This plasmid was generated by Dr Philipp Kaiser (NMI Tübingen) JOURNAL Distributed by ChromoTek GmbH, Martinsried, Germany. https://www.chromotek.com FEATURES Location/Qualifiers Promoter 1..19 /Imported_from="Pla sMapper" /X-Transferred_From="T7 prom" /X-Transferred_Similarity="100.00%" /Transferred_From="OTH0001_pSpot1" /Transferred_Similarity="100.00%" /label="T7 prom" misc_feature 19..46 /Imported_from="Pla sMapper" /X-Transferred_From="lacO reg" /X-Transferred_Similarity="100.00%" /Transferred_From="OTH0001_pSpot1" /Transferred_Similarity="100.00%" /label="lacO reg" /note="Geneious type: Regulatory Sequence" gene 64..80 /Imported_from="Pla sMapper" /X-Transferred_From="T7_transl_en_RBS" /X-Transferred_Similarity="100.00%" /modified_by="c.linkewinnebeck" /Transferred_From="OTH0001_pSpot1" /Transferred_Similarity="100.00%" /label="RBS" misc_feature 90..95 /Recognition_pattern="G^GATCC" /annotation_group="GGATCC" /label="BamHI" /note="Geneious type: restriction site" misc_feature 96..101 /Recognition_pattern="G^AATTC" /annotation_group="GAATTC" /label="EcoRI" /note="Geneious type: restriction site" misc_feature 102..107 /Recognition_pattern="GAGCT^C" /annotation_group="GAGCTC" /label="SacI" /note="Geneious type: restriction site" misc_feature 109..114 /Recognition_pattern="G^TCGAC" /annotation_group="GTCGAC" /label="SalI" /note="Geneious type: restriction site" misc_feature 115..120 /Recognition_pattern="A^AGCTT" /annotation_group="AAGCTT" /label="HindIII" /note="Geneious type: restriction site" misc_feature 121..128 /Recognition_pattern="GC^GGCCGC" /annotation_group="GCGGCCGC" /label="NotI" /note="Geneious type: restriction site" misc_feature 130..135 /Recognition_pattern="C^TCGAG" /annotation_group="CTCGAG" /label="XhoI" /note="Geneious type: restriction site" misc_feature 135..140 /Recognition_pattern="G^CTAGC" /annotation_group="GCTAGC" /label="NheI" /note="Geneious type: restriction site" CDS 141..176 /ApEinfo_fwdcolor="#ff8040" /ApEinfo_revcolor="#ff8040" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /Color="#ff0000" /modified_by="c.linkewinnebeck" /X-Transferred_From="Spot-Tag" /X-Transferred_Similarity="100.00%" /Transferred_From="OTH0001_pSpot1" /Transferred_Similarity="100.00%" /label="Spot-Tag" gene 177..179 /created_by="c.linkewinnebeck" /label="Stop" Terminator 361..518 /Imported_from="Pla sMapper" /X-Transferred_From="rrnB term" /X-Transferred_Similarity="100.00%" /Transferred_From="OTH0001_pSpot1" /Transferred_Similarity="100.00%" /label="rrnB term" Marker 798..1613 /Imported_from="Pla sMapper" /X-Transferred_From="kan2 marker" /X-Transferred_Similarity="99.75%" /modified_by="c.linkewinnebeck" /Transferred_From="OTH0001_pSpot1" /Transferred_Similarity="100.00%" /label="KanR" misc_feature 1781..2400 /Imported_from="Pla sMapper" /X-Transferred_From="pBR322 origin" /X-Transferred_Similarity="99.68%" /Transferred_From="OTH0001_pSpot1" /Transferred_Similarity="100.00%" /label="pBR322 origin" /note="Geneious type: Origin of Replication" ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt 61 tgtttaactt taagaaggag atataccatg gatccgaatt cgagctccgt cgacaagctt 121 gcggccgcac tcgagctagc ccggatcgcg tgcgcgcagt ctctcactgg agcagctaag 181 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat 241 aactagcata accccttggg gcaaacggtc tccagcttgg ctgttttggc ggatgagaga 301 agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt 361 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac 421 gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat 481 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 541 gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa 601 cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag 661 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctctttttgt ttatttttct 721 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 781 attgaaaaag gaagagtatg agccatattc aacgggaaac gtcttgctct aggccgcgat 841 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc 901 aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga 961 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc 1021 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat 1081 ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg 1141 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc 1201 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac 1261 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg 1321 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca 1381 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta 1441 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact 1501 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata 1561 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taataagcat 1621 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 1681 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 1741 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1801 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1861 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1921 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1981 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 2041 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 2101 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 2161 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 2221 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 2281 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 2341 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 2401 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgcgcaa cgcaattaat 2461 gtaagttagc gcgaattgat ctggtttgac agcttatcat cgactgcacg gtgcaccaat 2521 gcttctggcg tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact 2581 gcataattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat 2641 cataacggtt ctggcaaata ttctgaaatg agc //