LOCUS pSpot3 2969 bp DNA circular UNA 18-OCT-2017 DEFINITION Expression plasmid for C-term. Spot-tag fusion proteins, E. coli. ACCESSION ev-3 KEYWORDS . SOURCE ORGANISM . REFERENCE 1 AUTHORS This plasmid was generated by Dr Philipp Kaiser (NMI Tübingen) JOURNAL Distributed by ChromoTek GmbH, Martinsried, Germany. https://www.chromotek.com FEATURES Location/Qualifiers Promoter 1..19 /Imported_from="Pla sMapper" /Transferred_From="T7 prom" /Transferred_Similarity="100.00%" /label="T7 prom" misc_feature 19..46 /Imported_from="Pla sMapper" /Transferred_From="lacO reg" /Transferred_Similarity="100.00%" /label="lacO reg" /note="Geneious type: Regulatory Sequence" gene 64..80 /Imported_from="Pla sMapper" /Transferred_From="T7_transl_en_RBS" /Transferred_Similarity="100.00%" /modified_by="c.linkewinnebeck" /label="RBS" misc_feature 86..91 /Recognition_pattern="C^CATGG" /annotation_group="CCATGG" /label="NcoI" /note="Geneious type: restriction site" misc_feature 90..95 /Recognition_pattern="G^GATCC" /annotation_group="GGATCC" /label="BamHI" /note="Geneious type: restriction site" misc_feature 96..101 /Recognition_pattern="G^AATTC" /annotation_group="GAATTC" /label="EcoRI" /note="Geneious type: restriction site" misc_feature 102..107 /Recognition_pattern="GAGCT^C" /annotation_group="GAGCTC" /label="SacI" /note="Geneious type: restriction site" misc_feature 109..114 /Recognition_pattern="G^TCGAC" /annotation_group="GTCGAC" /label="SalI" /note="Geneious type: restriction site" misc_feature 115..120 /Recognition_pattern="A^AGCTT" /annotation_group="AAGCTT" /label="HindIII" /note="Geneious type: restriction site" misc_feature 121..128 /Recognition_pattern="GC^GGCCGC" /annotation_group="GCGGCCGC" /label="NotI" /note="Geneious type: restriction site" misc_feature 130..135 /Recognition_pattern="C^TCGAG" /annotation_group="CTCGAG" /label="XhoI" /note="Geneious type: restriction site" misc_feature 135..140 /Recognition_pattern="G^CTAGC" /annotation_group="GCTAGC" /label="NheI" /note="Geneious type: restriction site" CDS 141..176 /ApEinfo_fwdcolor="#ff8040" /ApEinfo_revcolor="#ff8040" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" /Color="#ff0000" /modified_by="c.linkewinnebeck" /Transferred_From="Spot-Tag" /Transferred_Similarity="100.00%" /label="Spot-Tag" gene 177..179 /created_by="c.linkewinnebeck" /label="Stop" Terminator 361..507 /Imported_from="Pla sMapper" /Transferred_From="rrnB term" /Transferred_Similarity="100.00%" /label="rrnB term" Marker 955..1815 /Imported_from="Pla sMapper" /Transferred_From="amp marker" /Transferred_Similarity="99.88%" /modified_by="c.linkewinnebeck" /label="AmpR" misc_feature 1970..2589 /Imported_from="Pla sMapper" /Transferred_From="pBR322 origin" /Transferred_Similarity="99.52%" /label="pBR322 origin" /note="Geneious type: Origin of Replication" misc_feature 2895..2922 /Imported_from="Pla sMapper" /Transferred_From="lacO reg" /Transferred_Similarity="82.14%" /label="lacO reg" /note="Geneious type: Regulatory Sequence" ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt 61 tgtttaactt taagaaggag atataccatg gatccgaatt cgagctccgt cgacaagctt 121 gcggccgcac tcgagctagc ccggatcgcg tgcgcgcagt ctctcactgg agcagctaag 181 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat 241 aactagcata accccttggg gcaaacggtc tccagcttgg ctgttttggc ggatgagaga 301 agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt 361 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac 421 gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat 481 caaataacag tcgaaagact gggcctttcg ttttatcgcc tgatgcggta ttttctcctt 541 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat 601 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct 661 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt 721 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta 781 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 841 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 901 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 961 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 1021 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 1081 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 1141 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 1201 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 1261 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 1321 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 1381 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 1441 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 1501 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 1561 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 1621 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 1681 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 1741 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 1801 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 1861 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 1921 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 1981 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 2041 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 2101 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 2161 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 2221 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 2281 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 2341 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 2401 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 2461 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 2521 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 2581 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 2641 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 2701 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 2761 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 2821 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 2881 cattaggcac cccaggcaat tgtgagcgga taacaatttg ttttttgcgc cgacatcata 2941 acggttctgg caaatattct gaaatgagc //