LOCUS pSpot5 7813 bp DNA circular UNA 16-OCT-2017 DEFINITION Expression plasmid for N-term. Spot-tag fusion proteins, S. cerevisiae ACCESSION ev-5 VERSION KEYWORDS . SOURCE ORGANISM REFERENCE 1 AUTHORS This plasmid was generated by Dr Philipp Kaiser (NMI Tübingen) JOURNAL Distributed by ChromoTek GmbH, Martinsried, Germany. https://www.chromotek.com FEATURES Location/Qualifiers misc_feature 1..6 /Recognition_pattern="G^AATTC" /annotation_group="GAATTC" /label="EcoRI" /note="Geneious type: restriction site" gene 10..12 /created_by="c.linkewinnebeck" /label="Start" gene 13..48 /Color="#ff0000" /created_by="c.linkewinnebeck" /label="Spot-Tag" misc_feature 47..52 /Recognition_pattern="CTGCA^G" /annotation_group="CTGCAG" /label="PstI" /note="Geneious type: restriction site" misc_feature 53..58 /Recognition_pattern="G^GATCC" /annotation_group="GGATCC" /label="BamHI" /note="Geneious type: restriction site" misc_feature 58..63 /Recognition_pattern="C^CTAGG" /annotation_group="CCTAGG" /label="AvrII" /note="Geneious type: restriction site" misc_feature 65..70 /Recognition_pattern="GGGCC^C" /annotation_group="GGGCCC" /label="ApaI" /note="Geneious type: restriction site" misc_feature 71..76 /Recognition_pattern="A^AGCTT" /annotation_group="AAGCTT" /label="HindIII" /note="Geneious type: restriction site" misc_feature 78..83 /Recognition_pattern="GAT^ATC" /annotation_group="GATATC" /label="EcoRV" /note="Geneious type: restriction site" misc_feature 86..91 /Recognition_pattern="C^TCGAG" /annotation_group="CTCGAG" /label="XhoI" /note="Geneious type: restriction site" misc_feature 93..98 /Recognition_pattern="C^CCGGG" /annotation_group="CCCGGG" /label="XmaI" /note="Geneious type: restriction site" misc_feature 100..105 /Recognition_pattern="G^CTAGC" /annotation_group="GCTAGC" /label="NheI" /note="Geneious type: restriction site" misc_feature 107..112 /Recognition_pattern="GAGCT^C" /annotation_group="GAGCTC" /label="SacI" /note="Geneious type: restriction site" Promoter complement(230..248) /Imported_from="Pla sMapper" /Transferred_From="T7 prom" /Transferred_Similarity="100.00%" /label="T7 prom" Reporter 252..412 /Imported_from="Pla sMapper" /Transferred_From="lacZ_a reporter" /Transferred_Similarity="99.38%" /label="lacZ_a reporter" misc_feature 429..735 /Imported_from="Pla sMapper" /Transferred_From="f1 origin" /Transferred_Similarity="99.67%" /label="f1 origin" /note="Geneious type: Origin of Replication" Marker 1564..2670 /Imported_from="Pla sMapper" /Transferred_From="LEU2 marker" /Transferred_Similarity="98.55%" /label="LEU2 marker" Gene complement(3398..3916) /Imported_from="Pla sMapper" /Transferred_From="CEN6_ARS4" /Transferred_Similarity="100.00%" /label="CEN6_ARS4" Marker 4048..4908 /Imported_from="Pla sMapper" /Transferred_From="amp marker" /Transferred_Similarity="99.88%" /modified_by="c.linkewinnebeck" /label="AmpR" misc_feature 5063..5682 /Imported_from="Pla sMapper" /Transferred_From="pBR322 origin" /Transferred_Similarity="99.52%" /label="pBR322 origin" /note="Geneious type: Origin of Replication" promoter 7239..9 /note="TPI1" /Transferred_From="TPI1 promoter" /Transferred_Similarity="98.80%" /label="TPI1 promoter" ORIGIN 1 gaattcacca tgccagatag agttagagct gtttctcatt ggtcttctgc agggatccct 61 aggtgggccc aagctttgat atccgctcga gccccgggtg ctagcggagc tcattaacct 121 agctaactga ataaggaaca atgaacgttt ttcctttctc ttgttcctag tattaatgac 181 tgaccgatac atcccttttt ttttttgtct ttgtctagct ccaattcgcc ctatagtgag 241 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 301 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 361 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct 421 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 481 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 541 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 601 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 661 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 721 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 781 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 841 ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg 901 cggtatttca caccgcatat cgaccggtcg aggagaactt ctagtatatc tacataccta 961 atattattgc cttattaaaa atggaatccc aacaattaca tcaaaatcca cattctcttc 1021 aaaatcaatt gtcctgtact tccttgttca tgtgtgttca aaaacgttat atttatagga 1081 taattatact ctatttctca acaagtaatt ggttgtttgg ccgagcggtc taaggcgcct 1141 gattcaagaa atatcttgac cgcagttaac tgtgggaata ctcaggtatc gtaagatgca 1201 agagttcgaa tctcttagca accattattt ttttcctcaa cataacgaga acacacaggg 1261 gcgctatcgc acagaatcaa attcgatgac tggaaatttt ttgttaattt cagaggtcgc 1321 ctgacgcata tacctttttc aactgaaaaa ttgggagaaa aaggaaaggt gagagcgccg 1381 gaaccggctt ttcatataga atagagaagc gttcatgact aaatgcttgc atcacaatac 1441 ttgaagttga caatattatt taaggaccta ttgttttttc caataggtgg ttagcaatcg 1501 tcttactttc taacttttct taccttttac atttcagcaa tatatatata tatatttcaa 1561 ggatatacca ttctaatgtc tgcccctaag aagatcgtcg ttttgccagg tgaccacgtt 1621 ggtcaagaaa tcacagccga agccattaag gttcttaaag ctatttctga tgttcgttcc 1681 aatgtcaagt tcgatttcga aaatcattta attggtggtg ctgctatcga tgctacaggt 1741 gttccacttc cagatgaggc gctggaagcc tccaagaagg ctgatgccgt tttgttaggt 1801 gctgtgggtg gtcctaaatg gggtaccggt agtgttagac ctgaacaagg tttactaaaa 1861 atccgtaaag aacttcaatt gtacgccaac ttaagaccat gtaactttgc atccgactct 1921 cttttagact tatctccaat caagccacaa tttgctaaag gtactgactt cgttgttgtc 1981 agagaattag tgggaggtat ttactttggt aagagaaagg aagacgatgg tgatggtgtc 2041 gcttgggata gtgaacaata caccgttcca gaagtgcaaa gaatcacaag aatggccgct 2101 ttcatggccc tacaacatga gccaccattg cctatttggt ccttggataa agctaatgtt 2161 ttggcctctt caagattatg gagaaaaact gtggaggaaa ccatcaagaa cgagttccct 2221 acattgaagg ttcaacatca attgattgat tctgccacca tgatcctagt taagaaccca 2281 acccacctaa atggtattat aatcaccagc aacatgtttg gtgacatcat ctccgatgaa 2341 gcctccgtta tcccaggttc cttgggtttg ttgccatctg cgtccttggc ctctttgcca 2401 gacaagaaca ccgcatttgg tttgtacgaa ccatgccacg gttctgctcc agatttgcca 2461 aagaataagg tcaaccctat cgccactatc ttgtctgctg caatgatgtt gaaattgtca 2521 ttgaacttgc ctgaagaagg taaggccatt gaagatgcag ttaaaaaggt tttggatgca 2581 ggtatcagaa ctggtgattt aggtggttcc aacagtacca ccgaagtcgg tgatgctgtc 2641 gccgaagaag ttaagaaaat ccttgcttaa aaagattctc tttttttatg atatttgtac 2701 ataaacttta taaatgaaat tcataataga aacgacacga aattacaaaa tggaatatgt 2761 tcatagggta gacgaaacta tatacgcaat ctacatacat ttatcaagaa ggagaaaaag 2821 gaggatgtaa aggaatacag gtaagcaaat tgatactaat ggctcaacgt gataaggaaa 2881 aagaattgca ctttaacatt aatattgaca aggaggaggg caccacacaa aaagttaggt 2941 gtaacagaaa atcatgaaac tatgattcct aatttatata ttggaggatt ttctctaaaa 3001 aaaaaaaaat acaacaaata aaaaacactc aatgacctga ccatttgatg gagtttaagt 3061 caataccttc ttgaaccatt tcccataatg gtgaaagttc cctcaagaat tttactctgt 3121 cagaaacggc cttaacgacg tagtcgatat ggtgcactct cagtacaatc tgctctgatg 3181 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt 3241 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc 3301 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat 3361 ttttataggt taatgtcatg ataataatgg tttcttagga cggatcgctt gcctgtaact 3421 tacacgcgcc tcgtatcttt taatgatgga ataatttggg aatttactct gtgtttattt 3481 atttttatgt tttgtatttg gattttagaa agtaaataaa gaaggtagaa gagttacgga 3541 atgaagaaaa aaaaataaac aaaggtttaa aaaatttcaa caaaaagcgt actttacata 3601 tatatttatt agacaagaaa agcagattaa atagatatac attcgattaa cgataagtaa 3661 aatgtaaaat cacaggattt tcgtgtgtgg tcttctacac agacaagatg aaacaattcg 3721 gcattaatac ctgagagcag gaagagcaag ataaaaggta gtatttgttg gcgatccccc 3781 tagagtcttt tacatcttcg gaaaacaaaa actatttttt ctttaatttc tttttttact 3841 ttctattttt aatttatata tttatattaa aaaatttaaa ttataattat ttttatagca 3901 cgtgatgaaa aggacccagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 3961 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 4021 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 4081 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 4141 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 4201 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 4261 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 4321 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 4381 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 4441 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 4501 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 4561 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 4621 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 4681 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 4741 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 4801 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 4861 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 4921 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 4981 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 5041 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 5101 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 5161 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 5221 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 5281 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 5341 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 5401 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 5461 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 5521 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 5581 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 5641 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 5701 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 5761 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 5821 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 5881 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt 5941 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 6001 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac 6061 agctatgacc atgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg 6121 gtaccgggcc ggccgtcgag cttgatggca tcgtggtgtc acgctcgtcg tttggtatgg 6181 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 6241 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 6301 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 6361 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 6421 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa 6481 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 6541 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 6601 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 6661 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 6721 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 6781 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 6841 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattgg 6901 ggatctacgt atggtcattt cttcttcaga ttccctcatg gagaaagtgc ggcagatgta 6961 tatgacagag tcgccagttt ccaagagact ttattcaggc acttccatga taggcaagag 7021 agaagaccca gagatgttgt tgtcctagtt acacatggta tttattccag agtattcctg 7081 atgaaatggt ttagatggac atacgaagag tttgaatcgt ttaccaatgt tcctaacggg 7141 agcgtaatgg tgatggaact ggacgaatcc atcaatagat acgtcctgag gaccgtgcta 7201 cccaaatgga ctgattgtga gggagaccta actacatagt gtttaaagat tacggatatt 7261 taacttactt agaataatgc catttttttg agttataata atcctacgtt agtgtgagcg 7321 ggatttaaac tgtgaggacc ttaatacatt cagacacttc tgcggtatca ccctacttat 7381 tcccttcgag attatatcta ggaacccatc aggttggtgg aagattaccc gttctaagac 7441 ttttcagctt cctctattga tgttacacct ggacacccct tttctggcat ccagttttta 7501 atcttcagtg gcatgtgaga ttctccgaaa ttaattaaag caatcacaca attctctcgg 7561 ataccacctc ggttgaaact gacaggtggt ttgttacgca tgctaatgca aaggagccta 7621 tatacctttg gctcggctgc tgtaacaggg aatataaagg gcagcataat ttaggagttt 7681 agtgaacttg caacatttac tattttccct tcttacgtaa atatttttct ttttaattct 7741 aaatcaatct ttttcaattt tttgtttgta ttcttttctt gcttaaatct ataactacaa 7801 aaaacacata cag //