LOCUS pSpot6 7806 bp DNA circular UNA 16-OCT-2017 DEFINITION Expression plasmid for C-term. Spot-tag fusion proteins, S. cerevisiae ACCESSION ev-6 VERSION KEYWORDS . SOURCE ORGANISM REFERENCE 1 AUTHORS This plasmid was generated by Dr Philipp Kaiser (NMI Tübingen) JOURNAL Distributed by ChromoTek GmbH, Martinsried, Germany. https://www.chromotek.com . FEATURES Location/Qualifiers misc_feature 1..6 /Recognition_pattern="G^AATTC" /annotation_group="GAATTC" /label="EcoRI" /note="Geneious type: restriction site" misc_feature 14..19 /Recognition_pattern="G^GATCC" /annotation_group="GGATCC" /label="BamHI" /note="Geneious type: restriction site" misc_feature 19..24 /Recognition_pattern="C^CTAGG" /annotation_group="CCTAGG" /label="AvrII" /note="Geneious type: restriction site" misc_feature 26..31 /Recognition_pattern="GGGCC^C" /annotation_group="GGGCCC" /label="ApaI" /note="Geneious type: restriction site" misc_feature 32..37 /Recognition_pattern="A^AGCTT" /annotation_group="AAGCTT" /label="HindIII" /note="Geneious type: restriction site" misc_feature 39..44 /Recognition_pattern="GAT^ATC" /annotation_group="GATATC" /label="EcoRV" /note="Geneious type: restriction site" misc_feature 47..52 /Recognition_pattern="C^TCGAG" /annotation_group="CTCGAG" /label="XhoI" /note="Geneious type: restriction site" misc_feature 54..59 /Recognition_pattern="C^CCGGG" /annotation_group="CCCGGG" /label="XmaI" /note="Geneious type: restriction site" misc_feature 61..66 /Recognition_pattern="G^CTAGC" /annotation_group="GCTAGC" /label="NheI" /note="Geneious type: restriction site" misc_feature 68..73 /Recognition_pattern="GAGCT^C" /annotation_group="GAGCTC" /label="SacI" /note="Geneious type: restriction site" gene 73..108 /Color="#ff0000" /created_by="c.linkewinnebeck" /Transferred_From="OTH0006_pSpot5" /Transferred_Similarity="100.00%" /label="Spot-Tag" gene 109..111 /created_by="c.linkewinnebeck" /label="Stop" Promoter complement(223..241) /Imported_from="Pla sMapper" /X-Transferred_From="OTH0001_pSpot1; OTH0003_pSpot2; OTH0004_pSpot3; T7 prom" /X-Transferred_Similarity="100.00%" /Transferred_From="OTH0001_pSpot1, OTH0003_pSpot2, OTH0004_pSpot3, OTH0005_pSpot4, OTH0006_pSpot5" /Transferred_Similarity="100.00%" /label="T7 prom" Reporter 245..405 /Imported_from="Pla sMapper" /X-Transferred_From="lacZ_a reporter" /X-Transferred_Similarity="99.38%" /Transferred_From="OTH0006_pSpot5" /Transferred_Similarity="100.00%" /label="lacZ_a reporter" misc_feature 422..728 /Imported_from="Pla sMapper" /X-Transferred_From="f1 origin" /X-Transferred_Similarity="99.67%" /Transferred_From="OTH0006_pSpot5" /Transferred_Similarity="100.00%" /label="f1 origin" /note="Geneious type: Origin of Replication" Marker 1557..2663 /Imported_from="Pla sMapper" /X-Transferred_From="LEU2 marker" /X-Transferred_Similarity="98.55%" /Transferred_From="OTH0006_pSpot5" /Transferred_Similarity="100.00%" /label="LEU2 marker" Gene complement(3391..3909) /Imported_from="Pla sMapper" /X-Transferred_From="CEN6_ARS4" /X-Transferred_Similarity="100.00%" /Transferred_From="OTH0006_pSpot5" /Transferred_Similarity="100.00%" /label="CEN6_ARS4" Marker 4041..4901 /Imported_from="Pla sMapper" /X-Transferred_From="OTH0004_pSpot3; amp marker" /X-Transferred_Similarity="100.00%; 99.88%" /modified_by="c.linkewinnebeck" /Transferred_From="OTH0004_pSpot3, OTH0005_pSpot4, OTH0006_pSpot5" /Transferred_Similarity="100.00%" /label="AmpR" misc_feature 5056..5675 /Imported_from="Pla sMapper" /X-Transferred_From="OTH0001_pSpot1; OTH0003_pSpot2; OTH0004_pSpot3; pBR322 origin" /X-Transferred_Similarity="100.00% -> 99.84%; 99.52%; 100.00%; 99.68%" /Transferred_From="OTH0004_pSpot3, OTH0005_pSpot4, OTH0006_pSpot5, OTH0001_pSpot1, OTH0003_pSpot2" /Transferred_Similarity="100.00% -> 99.84%" /label="pBR322 origin" /note="Geneious type: Origin of Replication" promoter 7232..9 /note="TPI1" /X-Transferred_From="TPI1 promoter" /X-Transferred_Similarity="98.80%" /Transferred_From="OTH0006_pSpot5" /Transferred_Similarity="100.00%" /label="TPI1 promoter" ORIGIN 1 gaattcacca tggggatccc taggtgggcc caagctttga tatccgctcg agccccgggt 61 gctagcggag ctccagatag agttagagct gtttctcatt ggtcttctta actagctaac 121 tgaataagga acaatgaacg tttttccttt ctcttgttcc tagtattaat gactgaccga 181 tacatccctt tttttttttg tctttgtcta gctccaattc gccctatagt gagtcgtatt 241 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 301 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca 361 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg 421 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 481 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 541 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 601 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 661 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 721 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 781 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa 841 aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt 901 tcacaccgca tatcgaccgg tcgaggagaa cttctagtat atctacatac ctaatattat 961 tgccttatta aaaatggaat cccaacaatt acatcaaaat ccacattctc ttcaaaatca 1021 attgtcctgt acttccttgt tcatgtgtgt tcaaaaacgt tatatttata ggataattat 1081 actctatttc tcaacaagta attggttgtt tggccgagcg gtctaaggcg cctgattcaa 1141 gaaatatctt gaccgcagtt aactgtggga atactcaggt atcgtaagat gcaagagttc 1201 gaatctctta gcaaccatta tttttttcct caacataacg agaacacaca ggggcgctat 1261 cgcacagaat caaattcgat gactggaaat tttttgttaa tttcagaggt cgcctgacgc 1321 atataccttt ttcaactgaa aaattgggag aaaaaggaaa ggtgagagcg ccggaaccgg 1381 cttttcatat agaatagaga agcgttcatg actaaatgct tgcatcacaa tacttgaagt 1441 tgacaatatt atttaaggac ctattgtttt ttccaatagg tggttagcaa tcgtcttact 1501 ttctaacttt tcttaccttt tacatttcag caatatatat atatatattt caaggatata 1561 ccattctaat gtctgcccct aagaagatcg tcgttttgcc aggtgaccac gttggtcaag 1621 aaatcacagc cgaagccatt aaggttctta aagctatttc tgatgttcgt tccaatgtca 1681 agttcgattt cgaaaatcat ttaattggtg gtgctgctat cgatgctaca ggtgttccac 1741 ttccagatga ggcgctggaa gcctccaaga aggctgatgc cgttttgtta ggtgctgtgg 1801 gtggtcctaa atggggtacc ggtagtgtta gacctgaaca aggtttacta aaaatccgta 1861 aagaacttca attgtacgcc aacttaagac catgtaactt tgcatccgac tctcttttag 1921 acttatctcc aatcaagcca caatttgcta aaggtactga cttcgttgtt gtcagagaat 1981 tagtgggagg tatttacttt ggtaagagaa aggaagacga tggtgatggt gtcgcttggg 2041 atagtgaaca atacaccgtt ccagaagtgc aaagaatcac aagaatggcc gctttcatgg 2101 ccctacaaca tgagccacca ttgcctattt ggtccttgga taaagctaat gttttggcct 2161 cttcaagatt atggagaaaa actgtggagg aaaccatcaa gaacgagttc cctacattga 2221 aggttcaaca tcaattgatt gattctgcca ccatgatcct agttaagaac ccaacccacc 2281 taaatggtat tataatcacc agcaacatgt ttggtgacat catctccgat gaagcctccg 2341 ttatcccagg ttccttgggt ttgttgccat ctgcgtcctt ggcctctttg ccagacaaga 2401 acaccgcatt tggtttgtac gaaccatgcc acggttctgc tccagatttg ccaaagaata 2461 aggtcaaccc tatcgccact atcttgtctg ctgcaatgat gttgaaattg tcattgaact 2521 tgcctgaaga aggtaaggcc attgaagatg cagttaaaaa ggttttggat gcaggtatca 2581 gaactggtga tttaggtggt tccaacagta ccaccgaagt cggtgatgct gtcgccgaag 2641 aagttaagaa aatccttgct taaaaagatt ctcttttttt atgatatttg tacataaact 2701 ttataaatga aattcataat agaaacgaca cgaaattaca aaatggaata tgttcatagg 2761 gtagacgaaa ctatatacgc aatctacata catttatcaa gaaggagaaa aaggaggatg 2821 taaaggaata caggtaagca aattgatact aatggctcaa cgtgataagg aaaaagaatt 2881 gcactttaac attaatattg acaaggagga gggcaccaca caaaaagtta ggtgtaacag 2941 aaaatcatga aactatgatt cctaatttat atattggagg attttctcta aaaaaaaaaa 3001 aatacaacaa ataaaaaaca ctcaatgacc tgaccatttg atggagttta agtcaatacc 3061 ttcttgaacc atttcccata atggtgaaag ttccctcaag aattttactc tgtcagaaac 3121 ggccttaacg acgtagtcga tatggtgcac tctcagtaca atctgctctg atgccgcata 3181 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct 3241 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt 3301 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata 3361 ggttaatgtc atgataataa tggtttctta ggacggatcg cttgcctgta acttacacgc 3421 gcctcgtatc ttttaatgat ggaataattt gggaatttac tctgtgttta tttattttta 3481 tgttttgtat ttggatttta gaaagtaaat aaagaaggta gaagagttac ggaatgaaga 3541 aaaaaaaata aacaaaggtt taaaaaattt caacaaaaag cgtactttac atatatattt 3601 attagacaag aaaagcagat taaatagata tacattcgat taacgataag taaaatgtaa 3661 aatcacagga ttttcgtgtg tggtcttcta cacagacaag atgaaacaat tcggcattaa 3721 tacctgagag caggaagagc aagataaaag gtagtatttg ttggcgatcc ccctagagtc 3781 ttttacatct tcggaaaaca aaaactattt tttctttaat ttcttttttt actttctatt 3841 tttaatttat atatttatat taaaaaattt aaattataat tatttttata gcacgtgatg 3901 aaaaggaccc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 3961 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 4021 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 4081 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 4141 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 4201 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 4261 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 4321 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 4381 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 4441 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 4501 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 4561 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 4621 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 4681 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 4741 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 4801 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 4861 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 4921 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 4981 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 5041 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 5101 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 5161 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 5221 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 5281 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 5341 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 5401 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 5461 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 5521 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 5581 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 5641 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 5701 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 5761 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 5821 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 5881 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 5941 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 6001 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 6061 accatgatta cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctggtaccgg 6121 gccggccgtc gagcttgatg gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 6181 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 6241 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 6301 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 6361 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 6421 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct 6481 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 6541 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 6601 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 6661 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 6721 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 6781 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 6841 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat tggggatcta 6901 cgtatggtca tttcttcttc agattccctc atggagaaag tgcggcagat gtatatgaca 6961 gagtcgccag tttccaagag actttattca ggcacttcca tgataggcaa gagagaagac 7021 ccagagatgt tgttgtccta gttacacatg gtatttattc cagagtattc ctgatgaaat 7081 ggtttagatg gacatacgaa gagtttgaat cgtttaccaa tgttcctaac gggagcgtaa 7141 tggtgatgga actggacgaa tccatcaata gatacgtcct gaggaccgtg ctacccaaat 7201 ggactgattg tgagggagac ctaactacat agtgtttaaa gattacggat atttaactta 7261 cttagaataa tgccattttt ttgagttata ataatcctac gttagtgtga gcgggattta 7321 aactgtgagg accttaatac attcagacac ttctgcggta tcaccctact tattcccttc 7381 gagattatat ctaggaaccc atcaggttgg tggaagatta cccgttctaa gacttttcag 7441 cttcctctat tgatgttaca cctggacacc ccttttctgg catccagttt ttaatcttca 7501 gtggcatgtg agattctccg aaattaatta aagcaatcac acaattctct cggataccac 7561 ctcggttgaa actgacaggt ggtttgttac gcatgctaat gcaaaggagc ctatatacct 7621 ttggctcggc tgctgtaaca gggaatataa agggcagcat aatttaggag tttagtgaac 7681 ttgcaacatt tactattttc ccttcttacg taaatatttt tctttttaat tctaaatcaa 7741 tctttttcaa ttttttgttt gtattctttt cttgcttaaa tctataacta caaaaaacac 7801 atacag 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