LOCUS pSpot7 9488 bp DNA circular UNA 16-OCT-2017 DEFINITION Expression plasmid for N-term. Spot-tag fusion proteins, S. cerevisiae ACCESSION ev-7 VERSION KEYWORDS . SOURCE ORGANISM REFERENCE 1 AUTHORS This plasmid was generated by Dr Philipp Kaiser (NMI Tübingen) JOURNAL Distributed by ChromoTek GmbH, Martinsried, Germany. https://www.chromotek.com FEATURES Location/Qualifiers misc_feature 1..6 /Recognition_pattern="G^AATTC" /annotation_group="GAATTC" /label="EcoRI" /note="Geneious type: restriction site" gene 10..12 /created_by="c.linkewinnebeck" /label="Start" gene 13..48 /Color="#ff0000" /created_by="c.linkewinnebeck" /X-Transferred_From="OTH0006_pSpot5" /X-Transferred_Similarity="100.00%" /Transferred_From="OTH0006_pSpot5, OTH0007_pSpot6" /Transferred_Similarity="100.00%" /label="Spot-Tag" misc_feature 53..58 /Recognition_pattern="G^GATCC" /annotation_group="GGATCC" /label="BamHI" /note="Geneious type: restriction site" misc_feature 58..63 /Recognition_pattern="C^CTAGG" /annotation_group="CCTAGG" /label="AvrII" /note="Geneious type: restriction site" misc_feature 65..70 /Recognition_pattern="GGGCC^C" /annotation_group="GGGCCC" /label="ApaI" /note="Geneious type: restriction site" misc_feature 71..76 /Recognition_pattern="A^AGCTT" /annotation_group="AAGCTT" /label="HindIII" /note="Geneious type: restriction site" misc_feature 78..83 /Recognition_pattern="GAT^ATC" /annotation_group="GATATC" /label="EcoRV" /note="Geneious type: restriction site" misc_feature 86..91 /Recognition_pattern="C^TCGAG" /annotation_group="CTCGAG" /label="XhoI" /note="Geneious type: restriction site" misc_feature 93..98 /Recognition_pattern="C^CCGGG" /annotation_group="CCCGGG" /label="XmaI" /note="Geneious type: restriction site" misc_feature 100..105 /Recognition_pattern="G^CTAGC" /annotation_group="GCTAGC" /label="NheI" /note="Geneious type: restriction site" misc_feature 107..112 /Recognition_pattern="GAGCT^C" /annotation_group="GAGCTC" /label="SacI" /note="Geneious type: restriction site" Promoter complement(230..248) /Imported_from="Pla sMapper" /Transferred_From="T7 prom" /Transferred_Similarity="100.00%" /label="T7 prom" Reporter 252..412 /Imported_from="Pla sMapper" /Transferred_From="lacZ_a reporter" /Transferred_Similarity="99.38%" /label="lacZ_a reporter" misc_feature 429..735 /Imported_from="Pla sMapper" /Transferred_From="f1 origin" /Transferred_Similarity="99.67%" /label="f1 origin" /note="Geneious type: Origin of Replication" Marker 1564..2670 /Imported_from="Pla sMapper" /Transferred_From="LEU2 marker" /Transferred_Similarity="98.55%" /label="LEU2 marker" misc_feature 3396..4865 /Imported_from="Pla sMapper" /Transferred_From="2micron origin" /Transferred_Similarity="98.37%" /label="2micron origin" /note="Geneious type: Origin of Replication" Gene complement(4950..4997) /Imported_from="Pla sMapper" /Transferred_From="FRT" /Transferred_Similarity="100.00%" /label="FRT" Marker 5723..6583 /Imported_from="Pla sMapper" /Transferred_From="amp marker" /Transferred_Similarity="99.88%" /label="amp marker" misc_feature 6738..7357 /Imported_from="Pla sMapper" /Transferred_From="pBR322 origin" /Transferred_Similarity="99.52%" /label="pBR322 origin" /note="Geneious type: Origin of Replication" promoter 8914..9 /note="TPI1" /X-Transferred_From="OTH0006_pSpot5; TPI1 promoter" /X-Transferred_Similarity="100.00%; 98.80%" /Transferred_From="OTH0006_pSpot5, OTH0007_pSpot6" /Transferred_Similarity="100.00%" /label="TPI1 promoter" ORIGIN 1 gaattcacca tgccagatag agttagagct gtttctcatt ggtcttctgc agggatccct 61 aggtgggccc aagctttgat atccgctcga gccccgggtg ctagcggagc tcattaacct 121 agctaactga ataaggaaca atgaacgttt ttcctttctc ttgttcctag tattaatgac 181 tgaccgatac atcccttttt ttttttgtct ttgtctagct ccaattcgcc ctatagtgag 241 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 301 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 361 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct 421 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 481 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 541 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 601 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 661 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 721 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 781 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 841 ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg 901 cggtatttca caccgcatat cgaccggtcg aggagaactt ctagtatatc tacataccta 961 atattattgc cttattaaaa atggaatccc aacaattaca tcaaaatcca cattctcttc 1021 aaaatcaatt gtcctgtact tccttgttca tgtgtgttca aaaacgttat atttatagga 1081 taattatact ctatttctca acaagtaatt ggttgtttgg ccgagcggtc taaggcgcct 1141 gattcaagaa atatcttgac cgcagttaac tgtgggaata ctcaggtatc gtaagatgca 1201 agagttcgaa tctcttagca accattattt ttttcctcaa cataacgaga acacacaggg 1261 gcgctatcgc acagaatcaa attcgatgac tggaaatttt ttgttaattt cagaggtcgc 1321 ctgacgcata tacctttttc aactgaaaaa ttgggagaaa aaggaaaggt gagagcgccg 1381 gaaccggctt ttcatataga atagagaagc gttcatgact aaatgcttgc atcacaatac 1441 ttgaagttga caatattatt taaggaccta ttgttttttc caataggtgg ttagcaatcg 1501 tcttactttc taacttttct taccttttac atttcagcaa tatatatata tatatttcaa 1561 ggatatacca ttctaatgtc tgcccctaag aagatcgtcg ttttgccagg tgaccacgtt 1621 ggtcaagaaa tcacagccga agccattaag gttcttaaag ctatttctga tgttcgttcc 1681 aatgtcaagt tcgatttcga aaatcattta attggtggtg ctgctatcga tgctacaggt 1741 gttccacttc cagatgaggc gctggaagcc tccaagaagg ctgatgccgt tttgttaggt 1801 gctgtgggtg gtcctaaatg gggtaccggt agtgttagac ctgaacaagg tttactaaaa 1861 atccgtaaag aacttcaatt gtacgccaac ttaagaccat gtaactttgc atccgactct 1921 cttttagact tatctccaat caagccacaa tttgctaaag gtactgactt cgttgttgtc 1981 agagaattag tgggaggtat ttactttggt aagagaaagg aagacgatgg tgatggtgtc 2041 gcttgggata gtgaacaata caccgttcca gaagtgcaaa gaatcacaag aatggccgct 2101 ttcatggccc tacaacatga gccaccattg cctatttggt ccttggataa agctaatgtt 2161 ttggcctctt caagattatg gagaaaaact gtggaggaaa ccatcaagaa cgagttccct 2221 acattgaagg ttcaacatca attgattgat tctgccacca tgatcctagt taagaaccca 2281 acccacctaa atggtattat aatcaccagc aacatgtttg gtgacatcat ctccgatgaa 2341 gcctccgtta tcccaggttc cttgggtttg ttgccatctg cgtccttggc ctctttgcca 2401 gacaagaaca ccgcatttgg tttgtacgaa ccatgccacg gttctgctcc agatttgcca 2461 aagaataagg tcaaccctat cgccactatc ttgtctgctg caatgatgtt gaaattgtca 2521 ttgaacttgc ctgaagaagg taaggccatt gaagatgcag ttaaaaaggt tttggatgca 2581 ggtatcagaa ctggtgattt aggtggttcc aacagtacca ccgaagtcgg tgatgctgtc 2641 gccgaagaag ttaagaaaat ccttgcttaa aaagattctc tttttttatg atatttgtac 2701 ataaacttta taaatgaaat tcataataga aacgacacga aattacaaaa tggaatatgt 2761 tcatagggta gacgaaacta tatacgcaat ctacatacat ttatcaagaa ggagaaaaag 2821 gaggatgtaa aggaatacag gtaagcaaat tgatactaat ggctcaacgt gataaggaaa 2881 aagaattgca ctttaacatt aatattgaca aggaggaggg caccacacaa aaagttaggt 2941 gtaacagaaa atcatgaaac tatgattcct aatttatata ttggaggatt ttctctaaaa 3001 aaaaaaaaat acaacaaata aaaaacactc aatgacctga ccatttgatg gagtttaagt 3061 caataccttc ttgaaccatt tcccataatg gtgaaagttc cctcaagaat tttactctgt 3121 cagaaacggc cttaacgacg tagtcgatat ggtgcactct cagtacaatc tgctctgatg 3181 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt 3241 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc 3301 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat 3361 ttttataggt taatgtcatg ataataatgg tttcttagac gtgcggccgc tctagaacta 3421 gtggatcaat tccacggact atagactata ctagtatact ccgtctactg tacgatacac 3481 ttccgctcag gtccttgtcc tttaacgagg ccttaccact cttttgttac tctattgatc 3541 cagctcagca aaggcagtgt gatctaagat tctatcttcg cgatgtagta aaactagcta 3601 gaccgagaaa gagactagaa atgcaaaagg cacttctaca atggctgcca tcattattat 3661 ccgatgtgac gctgcagctt ctcaatgata ttcgaatacg ctttgaggag atacagccta 3721 atatccgaca aactgtttta cagatttacg atcgtacttg ttacccatca ttgaattttg 3781 aacatccgaa cctgggagtt ttccctgaaa cagatagtat atttgaacct gtataataat 3841 atatagtcta gcgctttacg gaagacaatg tatgtatttc ggttcctgga gaaactattg 3901 catctattgc ataggtaatc ttgcacgtcg catccccggt tcattttctg cgtttccatc 3961 ttgcacttca atagcatatc tttgttaacg aagcatctgt gcttcatttt gtagaacaaa 4021 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac 4081 agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa 4141 acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac 4201 agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt 4261 gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt 4321 ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg 4381 ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact 4441 ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg 4501 catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat 4561 agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta 4621 tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag 4681 ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga 4741 ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata 4801 tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg 4861 caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc 4921 agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac 4981 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag 5041 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat 5101 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc 5161 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg 5221 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa 5281 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatca tatgcatagt 5341 accgagaaac tagtgcgaag tagtgatcag gtattgctgt tatctgatga gtatacgttg 5401 tcctggccac ggcagaagca cgcttatcgc tccaatttcc cacaacatta gtcaactccg 5461 ttaggccctt cattgaaaga aatgaggtca tcaaatgtct tccaatgtga gattttgggc 5521 cattttttat agcaaagatt gaataaggcg catttttctt caaagctgcg gccgcactct 5581 cactagtacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 5641 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 5701 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 5761 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 5821 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 5881 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 5941 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 6001 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 6061 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 6121 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 6181 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 6241 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 6301 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 6361 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 6421 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 6481 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 6541 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 6601 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 6661 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 6721 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 6781 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 6841 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 6901 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 6961 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 7021 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 7081 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 7141 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 7201 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 7261 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 7321 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 7381 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 7441 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 7501 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 7561 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 7621 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 7681 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta 7741 tgaccatgat tacgccaagc tcgaaattaa ccctcactaa agggaacaaa agctggtacc 7801 gggccggccg tcgagcttga tggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 7861 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 7921 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 7981 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 8041 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 8101 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg 8161 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 8221 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 8281 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 8341 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 8401 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 8461 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 8521 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attggggatc 8581 tacgtatggt catttcttct tcagattccc tcatggagaa agtgcggcag atgtatatga 8641 cagagtcgcc agtttccaag agactttatt caggcacttc catgataggc aagagagaag 8701 acccagagat gttgttgtcc tagttacaca tggtatttat tccagagtat tcctgatgaa 8761 atggtttaga tggacatacg aagagtttga atcgtttacc aatgttccta acgggagcgt 8821 aatggtgatg gaactggacg aatccatcaa tagatacgtc ctgaggaccg tgctacccaa 8881 atggactgat tgtgagggag acctaactac atagtgttta aagattacgg atatttaact 8941 tacttagaat aatgccattt ttttgagtta taataatcct acgttagtgt gagcgggatt 9001 taaactgtga ggaccttaat acattcagac acttctgcgg tatcacccta cttattccct 9061 tcgagattat atctaggaac ccatcaggtt ggtggaagat tacccgttct aagacttttc 9121 agcttcctct attgatgtta cacctggaca ccccttttct ggcatccagt ttttaatctt 9181 cagtggcatg tgagattctc cgaaattaat taaagcaatc acacaattct ctcggatacc 9241 acctcggttg aaactgacag gtggtttgtt acgcatgcta atgcaaagga gcctatatac 9301 ctttggctcg gctgctgtaa cagggaatat aaagggcagc ataatttagg agtttagtga 9361 acttgcaaca tttactattt tcccttctta cgtaaatatt tttcttttta attctaaatc 9421 aatctttttc aattttttgt ttgtattctt ttcttgctta aatctataac tacaaaaaac 9481 acatacag //