LOCUS pSpot8 9481 bp DNA circular UNA 16-OCT-2017 DEFINITION Expression plasmid for C-term. Spot-tag fusion proteins, S. cerevisiae ACCESSION ev-8 VERSION KEYWORDS . SOURCE ORGANISM REFERENCE 1 AUTHORS This plasmid was generated by Dr Philipp Kaiser (NMI Tübingen) JOURNAL Distributed by ChromoTek GmbH, Martinsried, Germany. https://www.chromotek.com FEATURES Location/Qualifiers misc_feature 1..6 /Recognition_pattern="G^AATTC" /annotation_group="GAATTC" /label="EcoRI" /note="Geneious type: restriction site" misc_feature 14..19 /Recognition_pattern="G^GATCC" /annotation_group="GGATCC" /label="BamHI" /note="Geneious type: restriction site" misc_feature 19..24 /Recognition_pattern="C^CTAGG" /annotation_group="CCTAGG" /label="AvrII" /note="Geneious type: restriction site" misc_feature 26..31 /Recognition_pattern="GGGCC^C" /annotation_group="GGGCCC" /label="ApaI" /note="Geneious type: restriction site" misc_feature 32..37 /Recognition_pattern="A^AGCTT" /annotation_group="AAGCTT" /label="HindIII" /note="Geneious type: restriction site" misc_feature 39..44 /Recognition_pattern="GAT^ATC" /annotation_group="GATATC" /label="EcoRV" /note="Geneious type: restriction site" misc_feature 47..52 /Recognition_pattern="C^TCGAG" /annotation_group="CTCGAG" /label="XhoI" /note="Geneious type: restriction site" misc_feature 54..59 /Recognition_pattern="C^CCGGG" /annotation_group="CCCGGG" /label="XmaI" /note="Geneious type: restriction site" misc_feature 61..66 /Recognition_pattern="G^CTAGC" /annotation_group="GCTAGC" /label="NheI" /note="Geneious type: restriction site" misc_feature 68..73 /Recognition_pattern="GAGCT^C" /annotation_group="GAGCTC" /label="SacI" /note="Geneious type: restriction site" gene 73..108 /Color="#ff0000" /created_by="c.linkewinnebeck" /X-Transferred_From="OTH0006_pSpot5" /X-Transferred_Similarity="100.00%" /Transferred_From="OTH0006_pSpot5, OTH0007_pSpot6" /Transferred_Similarity="100.00%" /label="Spot-Tag" gene 109..111 /created_by="c.linkewinnebeck" /label="Stop" Promoter complement(223..241) /Imported_from="Pla sMapper" /Transferred_From="T7 prom" /Transferred_Similarity="100.00%" /label="T7 prom" Reporter 245..405 /Imported_from="Pla sMapper" /Transferred_From="lacZ_a reporter" /Transferred_Similarity="99.38%" /label="lacZ_a reporter" misc_feature 422..728 /Imported_from="Pla sMapper" /Transferred_From="f1 origin" /Transferred_Similarity="99.67%" /label="f1 origin" /note="Geneious type: Origin of Replication" Marker 1557..2663 /Imported_from="Pla sMapper" /Transferred_From="LEU2 marker" /Transferred_Similarity="98.55%" /label="LEU2 marker" misc_feature 3389..4858 /Imported_from="Pla sMapper" /Transferred_From="2micron origin" /Transferred_Similarity="98.37%" /label="2micron origin" /note="Geneious type: Origin of Replication" misc_feature 3665..3670 /Recognition_pattern="CTGCA^G" /annotation_group="CTGCAG" /label="PstI" /note="Geneious type: restriction site" Gene complement(4943..4990) /Imported_from="Pla sMapper" /Transferred_From="FRT" /Transferred_Similarity="100.00%" /label="FRT" Marker 5716..6576 /Imported_from="Pla sMapper" /Transferred_From="amp marker" /Transferred_Similarity="99.88%" /label="amp marker" misc_feature 6731..7350 /Imported_from="Pla sMapper" /Transferred_From="pBR322 origin" /Transferred_Similarity="99.52%" /label="pBR322 origin" /note="Geneious type: Origin of Replication" promoter 8907..9 /note="TPI1" /X-Transferred_From="OTH0006_pSpot5; TPI1 promoter" /X-Transferred_Similarity="100.00%; 98.80%" /Transferred_From="OTH0006_pSpot5, OTH0007_pSpot6" /Transferred_Similarity="100.00%" /label="TPI1 promoter" ORIGIN 1 gaattcacca tggggatccc taggtgggcc caagctttga tatccgctcg agccccgggt 61 gctagcggag ctccagatag agttagagct gtttctcatt ggtcttctta actagctaac 121 tgaataagga acaatgaacg tttttccttt ctcttgttcc tagtattaat gactgaccga 181 tacatccctt tttttttttg tctttgtcta gctccaattc gccctatagt gagtcgtatt 241 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 301 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca 361 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg 421 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 481 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 541 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 601 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 661 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 721 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 781 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa 841 aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt 901 tcacaccgca tatcgaccgg tcgaggagaa cttctagtat atctacatac ctaatattat 961 tgccttatta aaaatggaat cccaacaatt acatcaaaat ccacattctc ttcaaaatca 1021 attgtcctgt acttccttgt tcatgtgtgt tcaaaaacgt tatatttata ggataattat 1081 actctatttc tcaacaagta attggttgtt tggccgagcg gtctaaggcg cctgattcaa 1141 gaaatatctt gaccgcagtt aactgtggga atactcaggt atcgtaagat gcaagagttc 1201 gaatctctta gcaaccatta tttttttcct caacataacg agaacacaca ggggcgctat 1261 cgcacagaat caaattcgat gactggaaat tttttgttaa tttcagaggt cgcctgacgc 1321 atataccttt ttcaactgaa aaattgggag aaaaaggaaa ggtgagagcg ccggaaccgg 1381 cttttcatat agaatagaga agcgttcatg actaaatgct tgcatcacaa tacttgaagt 1441 tgacaatatt atttaaggac ctattgtttt ttccaatagg tggttagcaa tcgtcttact 1501 ttctaacttt tcttaccttt tacatttcag caatatatat atatatattt caaggatata 1561 ccattctaat gtctgcccct aagaagatcg tcgttttgcc aggtgaccac gttggtcaag 1621 aaatcacagc cgaagccatt aaggttctta aagctatttc tgatgttcgt tccaatgtca 1681 agttcgattt cgaaaatcat ttaattggtg gtgctgctat cgatgctaca ggtgttccac 1741 ttccagatga ggcgctggaa gcctccaaga aggctgatgc cgttttgtta ggtgctgtgg 1801 gtggtcctaa atggggtacc ggtagtgtta gacctgaaca aggtttacta aaaatccgta 1861 aagaacttca attgtacgcc aacttaagac catgtaactt tgcatccgac tctcttttag 1921 acttatctcc aatcaagcca caatttgcta aaggtactga cttcgttgtt gtcagagaat 1981 tagtgggagg tatttacttt ggtaagagaa aggaagacga tggtgatggt gtcgcttggg 2041 atagtgaaca atacaccgtt ccagaagtgc aaagaatcac aagaatggcc gctttcatgg 2101 ccctacaaca tgagccacca ttgcctattt ggtccttgga taaagctaat gttttggcct 2161 cttcaagatt atggagaaaa actgtggagg aaaccatcaa gaacgagttc cctacattga 2221 aggttcaaca tcaattgatt gattctgcca ccatgatcct agttaagaac ccaacccacc 2281 taaatggtat tataatcacc agcaacatgt ttggtgacat catctccgat gaagcctccg 2341 ttatcccagg ttccttgggt ttgttgccat ctgcgtcctt ggcctctttg ccagacaaga 2401 acaccgcatt tggtttgtac gaaccatgcc acggttctgc tccagatttg ccaaagaata 2461 aggtcaaccc tatcgccact atcttgtctg ctgcaatgat gttgaaattg tcattgaact 2521 tgcctgaaga aggtaaggcc attgaagatg cagttaaaaa ggttttggat gcaggtatca 2581 gaactggtga tttaggtggt tccaacagta ccaccgaagt cggtgatgct gtcgccgaag 2641 aagttaagaa aatccttgct taaaaagatt ctcttttttt atgatatttg tacataaact 2701 ttataaatga aattcataat agaaacgaca cgaaattaca aaatggaata tgttcatagg 2761 gtagacgaaa ctatatacgc aatctacata catttatcaa gaaggagaaa aaggaggatg 2821 taaaggaata caggtaagca aattgatact aatggctcaa cgtgataagg aaaaagaatt 2881 gcactttaac attaatattg acaaggagga gggcaccaca caaaaagtta ggtgtaacag 2941 aaaatcatga aactatgatt cctaatttat atattggagg attttctcta aaaaaaaaaa 3001 aatacaacaa ataaaaaaca ctcaatgacc tgaccatttg atggagttta agtcaatacc 3061 ttcttgaacc atttcccata atggtgaaag ttccctcaag aattttactc tgtcagaaac 3121 ggccttaacg acgtagtcga tatggtgcac tctcagtaca atctgctctg atgccgcata 3181 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct 3241 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt 3301 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata 3361 ggttaatgtc atgataataa tggtttctta gacgtgcggc cgctctagaa ctagtggatc 3421 aattccacgg actatagact atactagtat actccgtcta ctgtacgata cacttccgct 3481 caggtccttg tcctttaacg aggccttacc actcttttgt tactctattg atccagctca 3541 gcaaaggcag tgtgatctaa gattctatct tcgcgatgta gtaaaactag ctagaccgag 3601 aaagagacta gaaatgcaaa aggcacttct acaatggctg ccatcattat tatccgatgt 3661 gacgctgcag cttctcaatg atattcgaat acgctttgag gagatacagc ctaatatccg 3721 acaaactgtt ttacagattt acgatcgtac ttgttaccca tcattgaatt ttgaacatcc 3781 gaacctggga gttttccctg aaacagatag tatatttgaa cctgtataat aatatatagt 3841 ctagcgcttt acggaagaca atgtatgtat ttcggttcct ggagaaacta ttgcatctat 3901 tgcataggta atcttgcacg tcgcatcccc ggttcatttt ctgcgtttcc atcttgcact 3961 tcaatagcat atctttgtta acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa 4021 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg 4081 caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa 4141 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag 4201 aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac 4261 aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc 4321 ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt 4381 tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc 4441 ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc 4501 gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg 4561 atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta 4621 cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac 4681 tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag 4741 tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca 4801 gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt 4861 ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc 4921 ttttggtttt caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa 4981 taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca 5041 catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata tatatataca 5101 tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc gtctatttat 5161 gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg cggggtatcg 5221 tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct caattggatt 5281 agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatatgcat agtaccgaga 5341 aactagtgcg aagtagtgat caggtattgc tgttatctga tgagtatacg ttgtcctggc 5401 cacggcagaa gcacgcttat cgctccaatt tcccacaaca ttagtcaact ccgttaggcc 5461 cttcattgaa agaaatgagg tcatcaaatg tcttccaatg tgagattttg ggccattttt 5521 tatagcaaag attgaataag gcgcattttt cttcaaagct gcggccgcac tctcactagt 5581 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 5641 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 5701 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 5761 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 5821 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 5881 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 5941 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 6001 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 6061 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 6121 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 6181 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 6241 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 6301 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 6361 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 6421 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 6481 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 6541 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 6601 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 6661 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 6721 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 6781 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 6841 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 6901 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 6961 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 7021 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 7081 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 7141 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 7201 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 7261 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 7321 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 7381 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 7441 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 7501 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 7561 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 7621 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct 7681 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat 7741 gattacgcca agctcgaaat taaccctcac taaagggaac aaaagctggt accgggccgg 7801 ccgtcgagct tgatggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 7861 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 7921 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 7981 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 8041 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 8101 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 8161 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 8221 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 8281 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 8341 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 8401 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 8461 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 8521 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattgggg atctacgtat 8581 ggtcatttct tcttcagatt ccctcatgga gaaagtgcgg cagatgtata tgacagagtc 8641 gccagtttcc aagagacttt attcaggcac ttccatgata ggcaagagag aagacccaga 8701 gatgttgttg tcctagttac acatggtatt tattccagag tattcctgat gaaatggttt 8761 agatggacat acgaagagtt tgaatcgttt accaatgttc ctaacgggag cgtaatggtg 8821 atggaactgg acgaatccat caatagatac gtcctgagga ccgtgctacc caaatggact 8881 gattgtgagg gagacctaac tacatagtgt ttaaagatta cggatattta acttacttag 8941 aataatgcca tttttttgag ttataataat cctacgttag tgtgagcggg atttaaactg 9001 tgaggacctt aatacattca gacacttctg cggtatcacc ctacttattc ccttcgagat 9061 tatatctagg aacccatcag gttggtggaa gattacccgt tctaagactt ttcagcttcc 9121 tctattgatg ttacacctgg acaccccttt tctggcatcc agtttttaat cttcagtggc 9181 atgtgagatt ctccgaaatt aattaaagca atcacacaat tctctcggat accacctcgg 9241 ttgaaactga caggtggttt gttacgcatg ctaatgcaaa ggagcctata tacctttggc 9301 tcggctgctg taacagggaa tataaagggc agcataattt aggagtttag tgaacttgca 9361 acatttacta ttttcccttc ttacgtaaat atttttcttt ttaattctaa atcaatcttt 9421 ttcaattttt tgtttgtatt cttttcttgc ttaaatctat aactacaaaa aacacataca 9481 g //