LOCUS pSpot8 9481 bp DNA circular UNA 16-OCT-2017
DEFINITION Expression plasmid for C-term. Spot-tag fusion proteins, S. cerevisiae
ACCESSION ev-8
VERSION
KEYWORDS .
SOURCE
ORGANISM
REFERENCE 1
AUTHORS This plasmid was generated by Dr Philipp Kaiser (NMI Tübingen)
JOURNAL Distributed by ChromoTek GmbH, Martinsried, Germany.
https://www.chromotek.com
FEATURES Location/Qualifiers
misc_feature 1..6
/Recognition_pattern="G^AATTC"
/annotation_group="GAATTC"
/label="EcoRI"
/note="Geneious type: restriction site"
misc_feature 14..19
/Recognition_pattern="G^GATCC"
/annotation_group="GGATCC"
/label="BamHI"
/note="Geneious type: restriction site"
misc_feature 19..24
/Recognition_pattern="C^CTAGG"
/annotation_group="CCTAGG"
/label="AvrII"
/note="Geneious type: restriction site"
misc_feature 26..31
/Recognition_pattern="GGGCC^C"
/annotation_group="GGGCCC"
/label="ApaI"
/note="Geneious type: restriction site"
misc_feature 32..37
/Recognition_pattern="A^AGCTT"
/annotation_group="AAGCTT"
/label="HindIII"
/note="Geneious type: restriction site"
misc_feature 39..44
/Recognition_pattern="GAT^ATC"
/annotation_group="GATATC"
/label="EcoRV"
/note="Geneious type: restriction site"
misc_feature 47..52
/Recognition_pattern="C^TCGAG"
/annotation_group="CTCGAG"
/label="XhoI"
/note="Geneious type: restriction site"
misc_feature 54..59
/Recognition_pattern="C^CCGGG"
/annotation_group="CCCGGG"
/label="XmaI"
/note="Geneious type: restriction site"
misc_feature 61..66
/Recognition_pattern="G^CTAGC"
/annotation_group="GCTAGC"
/label="NheI"
/note="Geneious type: restriction site"
misc_feature 68..73
/Recognition_pattern="GAGCT^C"
/annotation_group="GAGCTC"
/label="SacI"
/note="Geneious type: restriction site"
gene 73..108
/Color="#ff0000"
/created_by="c.linkewinnebeck"
/X-Transferred_From="OTH0006_pSpot5"
/X-Transferred_Similarity="100.00%"
/Transferred_From="OTH0006_pSpot5, OTH0007_pSpot6"
/Transferred_Similarity="100.00%"
/label="Spot-Tag"
gene 109..111
/created_by="c.linkewinnebeck"
/label="Stop"
Promoter complement(223..241)
/Imported_from="Pla
sMapper"
/Transferred_From="T7 prom"
/Transferred_Similarity="100.00%"
/label="T7 prom"
Reporter 245..405
/Imported_from="Pla
sMapper"
/Transferred_From="lacZ_a reporter"
/Transferred_Similarity="99.38%"
/label="lacZ_a reporter"
misc_feature 422..728
/Imported_from="Pla
sMapper"
/Transferred_From="f1 origin"
/Transferred_Similarity="99.67%"
/label="f1 origin"
/note="Geneious type: Origin of Replication"
Marker 1557..2663
/Imported_from="Pla
sMapper"
/Transferred_From="LEU2 marker"
/Transferred_Similarity="98.55%"
/label="LEU2 marker"
misc_feature 3389..4858
/Imported_from="Pla
sMapper"
/Transferred_From="2micron origin"
/Transferred_Similarity="98.37%"
/label="2micron origin"
/note="Geneious type: Origin of Replication"
misc_feature 3665..3670
/Recognition_pattern="CTGCA^G"
/annotation_group="CTGCAG"
/label="PstI"
/note="Geneious type: restriction site"
Gene complement(4943..4990)
/Imported_from="Pla
sMapper"
/Transferred_From="FRT"
/Transferred_Similarity="100.00%"
/label="FRT"
Marker 5716..6576
/Imported_from="Pla
sMapper"
/Transferred_From="amp marker"
/Transferred_Similarity="99.88%"
/label="amp marker"
misc_feature 6731..7350
/Imported_from="Pla
sMapper"
/Transferred_From="pBR322 origin"
/Transferred_Similarity="99.52%"
/label="pBR322 origin"
/note="Geneious type: Origin of Replication"
promoter 8907..9
/note="TPI1"
/X-Transferred_From="OTH0006_pSpot5; TPI1 promoter"
/X-Transferred_Similarity="100.00%; 98.80%"
/Transferred_From="OTH0006_pSpot5, OTH0007_pSpot6"
/Transferred_Similarity="100.00%"
/label="TPI1 promoter"
ORIGIN
1 gaattcacca tggggatccc taggtgggcc caagctttga tatccgctcg agccccgggt
61 gctagcggag ctccagatag agttagagct gtttctcatt ggtcttctta actagctaac
121 tgaataagga acaatgaacg tttttccttt ctcttgttcc tagtattaat gactgaccga
181 tacatccctt tttttttttg tctttgtcta gctccaattc gccctatagt gagtcgtatt
241 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
301 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
361 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg
421 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
481 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
541 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
601 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
661 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
721 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
781 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
841 aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
901 tcacaccgca tatcgaccgg tcgaggagaa cttctagtat atctacatac ctaatattat
961 tgccttatta aaaatggaat cccaacaatt acatcaaaat ccacattctc ttcaaaatca
1021 attgtcctgt acttccttgt tcatgtgtgt tcaaaaacgt tatatttata ggataattat
1081 actctatttc tcaacaagta attggttgtt tggccgagcg gtctaaggcg cctgattcaa
1141 gaaatatctt gaccgcagtt aactgtggga atactcaggt atcgtaagat gcaagagttc
1201 gaatctctta gcaaccatta tttttttcct caacataacg agaacacaca ggggcgctat
1261 cgcacagaat caaattcgat gactggaaat tttttgttaa tttcagaggt cgcctgacgc
1321 atataccttt ttcaactgaa aaattgggag aaaaaggaaa ggtgagagcg ccggaaccgg
1381 cttttcatat agaatagaga agcgttcatg actaaatgct tgcatcacaa tacttgaagt
1441 tgacaatatt atttaaggac ctattgtttt ttccaatagg tggttagcaa tcgtcttact
1501 ttctaacttt tcttaccttt tacatttcag caatatatat atatatattt caaggatata
1561 ccattctaat gtctgcccct aagaagatcg tcgttttgcc aggtgaccac gttggtcaag
1621 aaatcacagc cgaagccatt aaggttctta aagctatttc tgatgttcgt tccaatgtca
1681 agttcgattt cgaaaatcat ttaattggtg gtgctgctat cgatgctaca ggtgttccac
1741 ttccagatga ggcgctggaa gcctccaaga aggctgatgc cgttttgtta ggtgctgtgg
1801 gtggtcctaa atggggtacc ggtagtgtta gacctgaaca aggtttacta aaaatccgta
1861 aagaacttca attgtacgcc aacttaagac catgtaactt tgcatccgac tctcttttag
1921 acttatctcc aatcaagcca caatttgcta aaggtactga cttcgttgtt gtcagagaat
1981 tagtgggagg tatttacttt ggtaagagaa aggaagacga tggtgatggt gtcgcttggg
2041 atagtgaaca atacaccgtt ccagaagtgc aaagaatcac aagaatggcc gctttcatgg
2101 ccctacaaca tgagccacca ttgcctattt ggtccttgga taaagctaat gttttggcct
2161 cttcaagatt atggagaaaa actgtggagg aaaccatcaa gaacgagttc cctacattga
2221 aggttcaaca tcaattgatt gattctgcca ccatgatcct agttaagaac ccaacccacc
2281 taaatggtat tataatcacc agcaacatgt ttggtgacat catctccgat gaagcctccg
2341 ttatcccagg ttccttgggt ttgttgccat ctgcgtcctt ggcctctttg ccagacaaga
2401 acaccgcatt tggtttgtac gaaccatgcc acggttctgc tccagatttg ccaaagaata
2461 aggtcaaccc tatcgccact atcttgtctg ctgcaatgat gttgaaattg tcattgaact
2521 tgcctgaaga aggtaaggcc attgaagatg cagttaaaaa ggttttggat gcaggtatca
2581 gaactggtga tttaggtggt tccaacagta ccaccgaagt cggtgatgct gtcgccgaag
2641 aagttaagaa aatccttgct taaaaagatt ctcttttttt atgatatttg tacataaact
2701 ttataaatga aattcataat agaaacgaca cgaaattaca aaatggaata tgttcatagg
2761 gtagacgaaa ctatatacgc aatctacata catttatcaa gaaggagaaa aaggaggatg
2821 taaaggaata caggtaagca aattgatact aatggctcaa cgtgataagg aaaaagaatt
2881 gcactttaac attaatattg acaaggagga gggcaccaca caaaaagtta ggtgtaacag
2941 aaaatcatga aactatgatt cctaatttat atattggagg attttctcta aaaaaaaaaa
3001 aatacaacaa ataaaaaaca ctcaatgacc tgaccatttg atggagttta agtcaatacc
3061 ttcttgaacc atttcccata atggtgaaag ttccctcaag aattttactc tgtcagaaac
3121 ggccttaacg acgtagtcga tatggtgcac tctcagtaca atctgctctg atgccgcata
3181 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
3241 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
3301 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
3361 ggttaatgtc atgataataa tggtttctta gacgtgcggc cgctctagaa ctagtggatc
3421 aattccacgg actatagact atactagtat actccgtcta ctgtacgata cacttccgct
3481 caggtccttg tcctttaacg aggccttacc actcttttgt tactctattg atccagctca
3541 gcaaaggcag tgtgatctaa gattctatct tcgcgatgta gtaaaactag ctagaccgag
3601 aaagagacta gaaatgcaaa aggcacttct acaatggctg ccatcattat tatccgatgt
3661 gacgctgcag cttctcaatg atattcgaat acgctttgag gagatacagc ctaatatccg
3721 acaaactgtt ttacagattt acgatcgtac ttgttaccca tcattgaatt ttgaacatcc
3781 gaacctggga gttttccctg aaacagatag tatatttgaa cctgtataat aatatatagt
3841 ctagcgcttt acggaagaca atgtatgtat ttcggttcct ggagaaacta ttgcatctat
3901 tgcataggta atcttgcacg tcgcatcccc ggttcatttt ctgcgtttcc atcttgcact
3961 tcaatagcat atctttgtta acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa
4021 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
4081 caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa
4141 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
4201 aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac
4261 aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc
4321 ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt
4381 tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc
4441 ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc
4501 gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg
4561 atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta
4621 cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac
4681 tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag
4741 tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca
4801 gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt
4861 ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc
4921 ttttggtttt caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa
4981 taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca
5041 catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata tatatataca
5101 tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc gtctatttat
5161 gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg cggggtatcg
5221 tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct caattggatt
5281 agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatatgcat agtaccgaga
5341 aactagtgcg aagtagtgat caggtattgc tgttatctga tgagtatacg ttgtcctggc
5401 cacggcagaa gcacgcttat cgctccaatt tcccacaaca ttagtcaact ccgttaggcc
5461 cttcattgaa agaaatgagg tcatcaaatg tcttccaatg tgagattttg ggccattttt
5521 tatagcaaag attgaataag gcgcattttt cttcaaagct gcggccgcac tctcactagt
5581 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
5641 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
5701 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
5761 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
5821 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
5881 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
5941 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
6001 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
6061 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
6121 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
6181 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
6241 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
6301 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
6361 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
6421 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
6481 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
6541 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
6601 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
6661 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
6721 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
6781 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
6841 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
6901 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
6961 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
7021 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
7081 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
7141 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
7201 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
7261 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
7321 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
7381 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
7441 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
7501 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
7561 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
7621 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
7681 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
7741 gattacgcca agctcgaaat taaccctcac taaagggaac aaaagctggt accgggccgg
7801 ccgtcgagct tgatggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
7861 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
7921 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
7981 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
8041 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
8101 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
8161 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
8221 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
8281 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
8341 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
8401 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
8461 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
8521 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattgggg atctacgtat
8581 ggtcatttct tcttcagatt ccctcatgga gaaagtgcgg cagatgtata tgacagagtc
8641 gccagtttcc aagagacttt attcaggcac ttccatgata ggcaagagag aagacccaga
8701 gatgttgttg tcctagttac acatggtatt tattccagag tattcctgat gaaatggttt
8761 agatggacat acgaagagtt tgaatcgttt accaatgttc ctaacgggag cgtaatggtg
8821 atggaactgg acgaatccat caatagatac gtcctgagga ccgtgctacc caaatggact
8881 gattgtgagg gagacctaac tacatagtgt ttaaagatta cggatattta acttacttag
8941 aataatgcca tttttttgag ttataataat cctacgttag tgtgagcggg atttaaactg
9001 tgaggacctt aatacattca gacacttctg cggtatcacc ctacttattc ccttcgagat
9061 tatatctagg aacccatcag gttggtggaa gattacccgt tctaagactt ttcagcttcc
9121 tctattgatg ttacacctgg acaccccttt tctggcatcc agtttttaat cttcagtggc
9181 atgtgagatt ctccgaaatt aattaaagca atcacacaat tctctcggat accacctcgg
9241 ttgaaactga caggtggttt gttacgcatg ctaatgcaaa ggagcctata tacctttggc
9301 tcggctgctg taacagggaa tataaagggc agcataattt aggagtttag tgaacttgca
9361 acatttacta ttttcccttc ttacgtaaat atttttcttt ttaattctaa atcaatcttt
9421 ttcaattttt tgtttgtatt cttttcttgc ttaaatctat aactacaaaa aacacataca
9481 g
//