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Q1 – What is the composition of the precast gels? Are they comparable to traditional Tris Glycine or Bis-Tris gels?
The FastGene precast gels are based on Bis-Tris and are 1mm thick.
Q2 – How do the precast gels perform with instable proteins? Are there special recommendations for the loading and buffer to run native gels to stabilize the protein next to the ones described in your manuals?
If the protein is heat sensitive, than the running condition need to be at 4°C. Our gel can only run acid protein und alkaline condition. We usually set the running buffer at pH 8.8, but this is only a suggested pH value, customers need to test and get their satisfied running pH. The running time may be long.
Q3 – Which buffer can I used for these gels?
For FastGene Gels it is necessary to use MOPS buffer. We offer this buffer as dry pellets in pouches- only mixing with water is necessary. This buffer is also included in the samples.
Q4 – Are your gels keratin free?
The gel is produced keratin free, but there are no QC data available.
Q5 – Do you recommend wet- or semi-dry-blotting for the Precast Gels?
We recommend to use the wet transfer, because we have seen better results compared to the semi-dry transfer. We usually add 10 % Methanol in the transfer buffer, for smaller proteins we will increase the Methanol concentration to 20 %.
Q6 – What is the pH of the stacking and separating part of the gel?
The stacking gel pH is 6.4.
Q7 – Which electrophoresis systems can the FastGene PAGE Precast Gels work on?
These gels are compatible with the Bio-Rad Mini-PROTEAN® II & 3, Bio-Rad Mini-PROTEAN® Tetra System, Hoefer Mighty Small (SE 250) II Mini Vertical Electrophoresis Unit, and Hoefer Tall Mighty Small (SE 260) II Deluxe Mini Vertical Electrophoresis Unit.