Fewer steps with better results
FastGene Block & Go is an effective blocking reagent that also boosts the signal for Western blot analysis. Its unique formulation combines the blocking step, the primary antibody binding step and the secondary antibody step into a single step — saving you time and effort. Saving time can come at the cost of performance, but not for Block & Go! This novel reagent significantly enhances the signal developed with either HRP (horseradish peroxidase) or AP (alkaline phosphatase) substrates. FastGene Block & Go is guaranteed to give your Western blot procedure the winning combination of high signal & low background in record time.
Where does the time saving come from?
By combining 3 steps into 1 (blocking + primary antibody + secondary antibody), a Western procedure can be reduced by about 2.5 h (1-step procedure) compared to conventional methods using dehydrated milk or BSA. See the details below for our 1-step and 2-step protocols.
Enhanced signal intensity
|Compatibility||Western blot analysis|
|Substrates||HRP (horseradish peroxidase) or AP (alkaline phosphatase)|
|Wash buffer compatibility||PBST or TBST|
|Storage||1 year at 4°C|